Improving tobacco (Nicotiana tabacum) suspension cells as a molecular farming platform: a protease knock down approach
Manoj Kumar Mandal
Hrsg.: Fraunhofer IME, Aachen
2011, 149 S., num. mostly col. illus., Softcover
Sprache: Englisch
Aachen, TU, Diss., 2011
Fraunhofer Verlag
ISBN 978-3-8396-0294-2

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Inhalt
The first endogenous matrix-metalloproteinase NtMMP1 from tobacco BY-2 cells was characterized in detail. Transgenic BY-2 cell lines expressing antisense-RNA against different endogenous proteases (aspartic, cysteine, matrix-metallo and serine) separately (single-antisense) or simultaneously (multi-antisense) was generated. The inhibition of endogenous protease(s) in those lines was verified and the stability of recombinant protein(s) was tested.

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